J. Am. Chem. Soc. 2017, 139, 1372–1375.
The Purse Lab has invented a new cytidine analogue that becomes vastly more fluorescent upon incorporation into double-stranded DNA. Remarkably, the fluorescence turn-on response requires correct base pairing with guanosine, allowing visual discrimination of matched sequences, and shown in the photo below (left to right: single stranded probe, matched duplex, mismatched duplex). This fluorescence turn-on response promises new applications in biophysics. Read the details here! Congratulations to Dillon, Kristine, Ray, and Melissa, the student authors who invented the 8-DEA-tC probe molecule and completed a detailed investigation of its properties!
